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The [H]thymidine incorporation was evaluated by scintillation counting by using a top count β-counter (Microbeta Plus; Wallac).
Immunoglobulin-producing cells were enumerated by immunofluorescent staining for IgA, IgG and IgM and counting by using confocal microscopy.
In all cases, measurement of proliferation through cell counting by using a Coulter Z2 instrument (as described in our previous publications [ 84]) yielded identical results.
The samples were placed in the bottom of 6-well culture plate and 2 × 10 cells/mL of HUVECs was added (cell counting by using a Coulter Counter ZM).
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It has not been shown that lessons in which children learn to count by using blocks translate to a better understanding of numbers than a more abstract approach would have achieved.
The number of migrated cells was counted by using light microscope at high magnification field (20×).
Cell numbers were counted by using an automated cell counter (Bio-Rad, USA).
Hoechst-stained nucleus was counted by using the "Analyze particles" tool in ImageJ software.
Bacterial colony was counted by using colony counter under optical microscope (×40).
The number of cracks in the gage length (80 mm) were manually counted by using a microscope on both sides of each specimen.
After treatment for 8 h, migrated cells on the lower membrane were fixed with 4% paraformaldehyde, stained with 0.5% crystal violet and counted by using Image J.
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