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Thanks to its flexible architecture and performance, the system is suitable in highly demanding photon counting applications based on SPAD arrays, as well as in many other scientific experiments.
The yield, sample-input requirements and amplification-free library prep of PacBio potentially make it unsuitable for counting applications and for applications involving significant prior enrichment such as exome sequencing [ 15] and ChIP-seq [ 18].
Provided the impulse response function does not change substantially over the time-course of a measurement as in the photon counting applications described herein, this initial investment may be easily justified by enabling subsequent high speed data analysis through simple digital filtering.
Examples of the use of potassium chloride as a tracer are given in the context of ion exchange and electrochemical migration studies, and comparisons in performance are drawn from literature with hyper pure germanium semiconductor detectors, which are more commonly utilised detectors in high-resolution counting applications.
This underlines the fact that sampling statistics can be used to greatly improve confidence in both variant calling and RNA transcript counting applications.
Fortunately, in photon counting applications such as those considered herein, both the random noise in the baseline and noise from variance in the peak heights of the transients are naturally removed through the counting process.
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Although this detector was designed primarily for imaging applications [21, 22] and has an 11 × 11 anode readout scheme, it provides the large detection volume required for the counting application developed here.
As for the other possible condition that might result in the false detection results, Figure 33(b) illustrated this kind of example and also showed the false detection consequence in the vehicle counting application.
From the testing results, we would also list two kinds of failed examples in Figure 33 to indicate some erroneous detected results in the vehicle counting application, which may be reasonable to illustrate the failed conditions in a more quantized manner.
The primary objective of this study was to develop a practical evaluation scheme to assess the efficacy and validity of an automatic cough counting application.
After 8 weeks of incubation, colonies were stained over night with 1 mg/mL p-iodonitrotetrazolium solution (Sigma) and scored using the colony counting application from the Quantity One software (Bio-Rad).
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