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We present a high-throughput label-free electrical-impedance spectroscopy (EIS) platform composed of a 96×9696 microelectrode array for counting and analysis of breast tumor cells (MCF-7).
Benefiting from the integration of microelectronic and microfluidic techniques, impedance sensing methods have expanded their applications to nearly all aspects of biology, including living cell counting and analysis, cell biology research, cancer research, drug screening, and food and environmental safety monitoring.
As brain tissues were sliced in series sections, the counting and analysis in cell number and structures were able to be done at least from two sections for each of barrels.
Reads aligning to the genome were then mapped against homology-based annotations using TASE (Tag counting and Analysis of Solexa Experiments), producing read-frequencies per annotation [ 56].
In brief, epididymides were dissected and placed in prewarmed M2 medium, minced, and incubated at 37°C for 30 min in a CO2 incubator prior to counting and analysis of sperm motility.
The investigator collecting the biopsy images and performing the analyses was blinded to experimental setup and a fraction of the biopsies were analysed twice to ensure consistency in counting and analysis.
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Terminal counts and analysis of dendrite morphology were performed manually.
PKY and NJ have carried out all the histological, immunohistochemical work, along with cell count and analysis of the images.
Cell counts and analysis of viability by trypan blue exclusion staining were performed using a ViCell XR counter (Beckman Coulter, Brea, CA, USA).
Colony factor unit counting and mycological analysis of mice vaginal mucosa after Grocott-Gomori methenamine-silver staining confirmed the absence of C. glabrata.
JS carried out the podocyte counting and morphometry analysis.
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