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Second, the much smaller seed size of A. thaliana may have promoted longer distance migration of its seeds in transported soil or other material, and the higher rate of gene flow could be countering selection.
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MacConkey agar plates containing tobramycin (8 μg/mL) were used for counter selection, and transconjugants were selected on colony form.
The transformants were selected by 5-FOA counter selection.
The transformants were selected by 5-Fluoroorotic Acid (5-FOA) counter selection.
Since RpsL is a streptomycin sensitive gene, the mutated BAC DNA was selected on the basis of increased streptomycin resistance using a Counter Selection Modification kit (Gene Bridges).
Perhaps if you ask extra nicely, there's an under-the-counter selection of exorbitantly priced hockey sticks, bespoke cricket bats, rigorously flame-proof shell suits and ethically sourced trainers.
Indeed, a meta analysis of Liliaceae indicated counter selection against large genomes in arid climates (Carta and Peruzzi 2016).
By counter selection against HeLa cells, aptamers that can distinguish ovarian cancer from cervical cancer were selected.
The sequences binding to HeLa cells were successfully removed by counter selection in subsequent rounds, while the enrichment towards the target cell line was maintained (Figure 1b).
In contrast to the level 0 modules, the level 1 destination vectors confer ampicillin resistance, allowing efficient counter selection against level 0 module backbones.
Alternatively, high-level expression of Venus may not be well tolerated in haematopoietic cells causing silencing of transgene expression over time or counter selection in stem cells.
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