Blood cell count was calculated by an electric cell counter (Coulter Counter Model CBC5; Coulter Electronics, Bath, UK).
Subsequently, the theoretical prediction of the counter defocus was calculated by the following equation [ 49]: where δ l is the pseudo focal shift from the focal plane, c is dioptric power, n is the refractive index, R is pupil radius, and Z 2 0 is the Zernike coefficient of defocus.
The radioactivity emitted from bound [3H]putrescine was quantitated by liquid scintillation using a Beckman LS6500 scintillation counter, and TG activity was calculated after background subtraction as fold increase over the radioactive reading obtained from the lysate of empty vector transfected cells.
Labeled acetylcholine was then extracted and counted in a liquid scintillation counter and the activity was calculated as nanomoles synthesized per hour per microgram DNA.
Everything he said before and during the final was calculated to counter the inevitable fear, with one message to the fore: they are history, you are the present, you will win.
The activity concentrations in 100 μL samples of both the aqueous and the organic phase were measured in a gamma counter, and the log Pow was calculated.
When Alexander Hamilton proposed the establishment of a central bank, Thomas Jefferson countered that Hamilton's system "was calculated to undermine and demolish the republic".
The radioactivity of the bound and unbound material was counted, using a γ-counter, and the immunoreactive percentage was calculated.
The radioactive content in the tissue of interest (blood, tumor, muscle, bone, liver, spleen, kidney, heart, lung, pancreas, brain, skin, small intestine, large intestine, stomach, tail, thyroid, and feces) was measured on a Wizard 2470 Automatic Gamma Counter and the tissue-associated activity was calculated as the mean %ID/g.
Suspension lines were counted using a Coulter Vi-Cell counter (Beckman Coulter Inc .. Growth inhibition was calculated as a function of the number of generations inhibited in the presence of PD 0332991 versus the number of generations over the same time course in the absence of PD 0332991.
