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The NGP was counted manually for five panicles.
The number of globular embryos was counted manually for representative smaller aliquots under a Leica Wild (M3Z) (Leica, Wetzlar, Germany) stereomicroscope.
The number of coughs was counted manually for 10 min from the start of inhalation of each provocation solution [ 13, 15].
The firing rate for each cell recorded for each stimulation parameter was counted manually for the full stimulus train and then averaged and plotted using GraphPad Prism statistical software (GraphPad Software Inc, La Jolla, CA).. Light-evoked spike amplitudes were measured for the duration of the full stimulus train.
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Dendrite ends were counted manually from stacked images for the entire field of view.
Nuclear γ-H2AX foci were then counted manually from at least 50 cells for each treatment condition by an investigator blinded to treatment conditions.
To count, cells were washed with Hanks, trypsinized, centrifuged, and resuspended in 5 ml of media and then counted manually using Haemocytometer every day for 7 days.
Colonies were counted manually after incubation at 30°C for 48 h with a detection limit of 0.5 log CFU/mL.
Their number was counted manually and electrical balance was used for dry weight of nodules.
The cells positive for MHC and PCNA were counted manually in all ventricles.
Each cover slip was divided into quadrants and the number of cells (total and positive for staining) were counted manually.
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