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Ca2+ sparks were counted manually and with a spark-counting software algorithm to verify the result objectively.
For determination of CFU the colonies of each dilution were counted manually and calculated as CFU/mL.
"If all the votes are counted manually and then if you keep reviewing it," said Senator Tom Harkin, an Iowa Democrat who is also an ardent Gore backer, "you could be called a sore loser".
For quantification of colocalisation, total cell numbers (nuclei) and cells which stained positive for COX-1, COX-2 or pro-SP-C were counted manually and percentages of cells which stained positive for respective markers were calculated for at least 5 different images from each mouse (n = 4).
For quantification of colocalization, total number of cells (nuclei) and cells which stained positive for COX-1, COX-2 or AQP5 were counted manually and percentages of cells which stained positive for according markers were calculated from at least 4 different images.
Number of nodules plant−1 were counted manually and their dry wt.
Similar(18)
Total number of starting cells per field were counted manually on imageJ and number of PI positive nuclei were counted automatically for each timepoint using an imageJ macro.
However, platelets were counted manually (light microscopy) and did not report additional hematologic features of the resulting PC.
After incubation for 9 h, the migrated cells were stained by hematoxylin and eosin and counted manually under a microscope at 50× magnification.
Total and blebbing cells were counted manually (ntotal ∼250 cells/condition and experiment).
Plates were stained with 0.005% crystal violet, and the colonies were counted manually under a microscope and photographed (Slivova et al, 2004).
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