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Shorter foraging periods would effectively cause an apparent decline in natality rates when simply counting ratios of adults to pups because more adults would be counted in relation to the number of pups in later years.
A counting grid was used and each subfield (50 μm × 50 μm) with positive staining for calcium noduli was counted in relation to the total numbers of subfields; the mean was used as a single value for analysis.
The numbers of propidium-iodide-, active-caspase-3 or TUNEL-positive cells were counted in relation to the number of neurons with apoptotic nuclear morphology as detected by Hoechst 33342 in the same areas within the same cultures.
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Stained cells were counted manually in relation to the total number of nuclei in the field.
Estimates obtained through traditional two-dimensional counting methods are heavily biased by the counting parameters in relation to the size and shape of the neurons to be counted, resulting in a large range of inaccurate counts.
The risk of double counting in relation to health, biodiversity or carbon sequestration is limited, as noted above.
"Ploidy C": ploidy determined from chromosome counting in relation to haploid number.
The seasonal variation in cardiovascular deaths [ 96] has indeed been linked to increased neutrophil count in relation to winter respiratory infections [ 116].
In persons without diabetes, studies looking for short-term changes in leukocyte counts in relation to air pollution have given inconclusive results.
For all statistical analyses conducted, the results for total sperm count in relation to metals were similar to those for sperm concentration, so they are not presented here.
However, that study did not evaluate the independent prognostic value of the Chalkley count in relation to the uPA or PAI-1 levels, because of an 'insufficient number of events for the number of variables' (Fox et al, 2001).
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