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Bone marrow cells were filtered, lysed with ACK solution (Lonza Biowhittaker) and counted for further immunofluorescent staining.
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We identified several disease categories (i.e., cardiovascular, gastrointestinal, heat-related, and respiratory) associated with climate change, and then selected corresponding ICD-9 codes with the highest hospitalization counts for further analyses.
We chose a cut off of 10.75 × 10/L for white cell count and 147.5 × 10/L for platelet count for further analysis.
But since the system did not provide the ability to set a certain threshold as background activity, a calculated T/B ratio was used instead of raw counts for further analysis.
But further down the list, the order counted for less.
"It counted for 3.
That counted for something.
Popular opinion counted for little.
That counted for a lot.
A total of 14267 genes were annotated using RefSeq database and the raw read counts for genes were generated for further downstream analyses.
Twenty-five cells were analyzed for each cell line, and locus-specific signals for SO (SEPT9) and Cy5 (HER2) were manually counted and plotted for further analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com