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Some of the seeds germinated on the agar plate and were counted as viable seeds.
In adherent cell cultures, only cells adhering to the culture dish before trypsinization were counted as viable.
Cell viability was calculated using the same images and methodology used for cell number assessment: nuclei surrounded by actin were counted as viable, while all other nuclei were considered to be dead cells.
For each time point and treatment, nuclei uniformly stained with Hoechst were counted as viable and condensed, and PI-positive nuclei were scored as dead neurons and expressed as a percentage of the total population, three subfields captured per well, with a minimum of three wells analyzed per condition.
Similar(56)
The viability of parasites was determined by direct observation of the cestode under the microscope, counting as viable the parasites that exhibited full motion during 1 minute of observation [ 8].
RD cells without being stained by trypan blue were counted as the viable cells.
All pixel values > x are counted as enhancing viable tumor, mean is the average pixel value from a sample region of normal adjacent background parenchyma, and std_dev is the SD from the same sample.
Non-fluorescing sperm moved their flagella (i.e., they were alive), and sperm that were green with red spots had possibly been injured in the staining solution [18]; thus, only entirely red sperm were counted as non-viable, and all others were considered viable.
The number of submerged seeds were counted and taken as viable.
A, Viable Cells were counted as PI and Annexin V double negative cells.
The viable and nonviable cells were counted as (2) Cell count = Number of cells × Dilution factor Area × Thickness of the liquid film.
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