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We investigated the influence of the number of BNCs counted on the calculated mean MN frequency by incremental counting of slides from a single blood culture.
The mean value of read count in sliding window determines whether the extension should continue or not.
For comparison, MIB-1 index was also determined by regular counting of MIB-1-stained MIB-1-stained MIB-1-stainedtinction between proliferating lymphocyteslidesproliferapplyingmor cells.
We calculated the FF by counting spikes in sliding windows of 10 ms for each cell and each of the five bins.
The length of ETC abnormality was determined by counting the number of slides the abnormality appeared in and multiplying by 10 μm.
Most programs use (sometimes implicitly) kernel smoothing to compensate for mapping ambiguities, the most popular kernel being the uniform density, which is equivalent to counting tags in sliding windows of fixed-length [ 8- 12].
In this setting, optimal discrimination between patients with and without malignancy was provided by a minimal total cell count of 5000 per slide, leading to relatively high overall inadequacy rates.
Hold for one count, then slide up.
To determine whether or not parasite infection was associated with the standardised WBC count, the number of slide views required to find 100 WBCs was used as the response variable (as 100 WBCs were counted for each bird), with the mean number of RBCs per slide view as a covariate in a GLM with quasipoisson error distributions.
As the average size of sequenced genomic DNA fragments was around 260 bp (see Additional file 1: Figure S16), to encompass tags from both ends of NFI binding sites, the tags were counted in a sliding window of 600 bp and we required a minimum distance of 600 bp ("vicinity range" parameters) between two NFI sites to avoid counting the same site multiple times.
A CNV was kept if the |log2ratio| of the counts of reads per sliding window was bigger than 1, and if the CNVs were called by at least 5 consecutive windows.
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