However, based on our counting of sections, it appears that our efficiency of isolating T cells from lung may be as low as 15 20%.
Assessment of chimerism in neocortex and the dentate gyrus was performed by cell counting of sections stained to detect nuclear DNA (Hoechst 33342) and LacZ enzyme activity.
To estimate the total number of axons/subjects, the number of axons counted on sections was multiplied by 6.
Total number of chondrocytes and positive-staining chondrocytes were counted in the femur and tibia, respectively, in three or more sections on 3 6 high-power fields (×400) according to the size of articular sections, and mean count of these sections was used to determine a proportion of cells expressing MMP-3.
He admitted attempting to cause grievous bodily harm with intent, two counts of section 20 assault, 10 other assaults, aggravated taking of a motor vehicle without consent and dangerous driving.
In Section 1, we reviewed two strategies for the allocation of samples to individual methods, balance heuristic with equal count of samples (Section 2.2.1) and non-balance heuristic with sample numbers proportional to the variance (Section 2.3).
Quantitative analysis of axons by manual counting of histological sections can be slow, which can limit the number of bridge designs evaluated.
Counting of lumbar sections of G1del mice aged 20 (n = 3) and 25 weeks (n = 3) indicated ATF3 expression in 40 ± 6 (25 62) % (Mean ± SE, range) and 54 ± 13 (37 75) % of motor neurons with Golgi fragmentation.
Counting of brain sections was conducted in the caudate-putamen area in a 1-in-10 series per animal, ranging from bregma +1.5 mm to −0.5 mm coronal coordinates.
Cell counting of five sections each showed that about 40% +/− 16.5% of all osteocytes in the cortical bone matrix of P17 tibiae were β-gal+, the majority of them being located at the endosteal side of the cortical bone shaft (supplementary material Fig. S3A).
To measure the depth of cell adhesions, we counted the number of sections (with at least a three-fold increase in phalloidin or paxillin signal over background) and multiplied this value by the step size between sections.
