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Because flow cytometry reveals genome size rather than a count of chromosomes, ploidy must be verified by chromosome counts in at least a few samples.
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Early cytological studies in switchgrass focused mainly on overall counts of chromosomes rather than their individual morphology and molecular structure [ 40].
Many early analyses of association between a binary phenotype and a genetic marker used simple tests for association in contingency tables in which cell entries were counts of chromosomes rather than people.
Chromosomal analysis was also performed on all lines by counting of chromosome spreads.
Subsequently, by combining the results of the de novo assembly and the chromosome copy number analysis, a genome map that comprised 35 different chromosomal structures and a total count of 68 chromosomes was established (Fig. 1).
Dr. Joe Hin Tjio, the research biologist who produced the first correct count of the chromosomes in human cells, died Nov. 27 in Gaithersburg, Md .He was 82.
The count of 18 chromosomes is consistent with the karyotype for a female L. geometricus that was previously described [14].
The African plantains are interesting in having a proportionally higher number of reads mapping to B_chr7 despite the low gene count of this chromosome.
Z-stacks were obtained using the ApoTome and images were displayed using maximum projection to count the number of chromosomes in oocytes.
We assessed the number of B chromosomes by two methods: counting CID foci in prometaphase I oocytes and direct counting of B chromosomes in larval neuroblasts by careful analysis of z-stacks from acquired images.
Given that C. sativa has a chromosome count of n = 20, chromosome splitting or fusion could then have increased the chromosomes from 18 to 20, or decreased the chromosomes from 21 or 24 to 20.
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