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Immunocytometry (Becton Dickinson FACS count Immunocytometry systems, California) determined the CD4 cell count in total blood using FACS count kits reagent (Becton Dickinson, BD Bioscience).
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Cell proliferation was determined by the cell count kit cell counting kit-8 (CCK-8, Dojindo, Shanghai, China).
CD4 count was measured using the Coulter Manual CD4 Count kit (Beckman Coulter, Hialeah, FL, USA).
The antiproliferative effect of Oridonin was assessed using Cell Count Kit-8 (Dojindo Molecular Technologies, Inc., Gaithersburg, MD).
Concentration of plasma insulin was analysed by radioimmunoassay using Coat-A-Count kits (DPC, Biermann GmbH, Bad Nauheim, Germany).
Cell Counting Kit-8.
Proliferation of cells was measured using a Cell Counting Kit.
Cell Counting Kit-8 (CCK-8) was used to perform the cell viability studies.
Cell counting kit-8 (CCK-8) was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan).
The cytotoxicity of the nanomachine was assessed by cell counting kit (CCK -8 assay (Beyotime, Beijing, CCK -8.
The cell counting kit-8 (CCK-8) was purchased from Dojindo laboratories (Kumamoto, Japan).
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