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The temporal relation between CD4 count determinations and concurrent illnesses was sometimes difficult to determine due to the retrospective nature of the study.
Cell growth was monitored by optical density measurements at 750 nm, cell count determinations and dry biomass determination.
Routine assessments also included CSF white blood cell (WBC) counts and peripheral blood CD4 cell count determinations.
A sample of 20 female fleas was collected immediately after the infectious blood meal, placed at −80°C, and subsequently used for CFU plate count determinations of the average infectious dose per flea.
CD4 cell count determinations were performed on a daily basis.
If he had CD4 cell count determinations during the first and second semesters, the patient was considered retained in care.
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NIR spectroscopy describes informative patterns in application for somatic cell count determination, distinguishing of healthy and mastitis milk as well as in detection the presence and growth of specific bacteria, based on milk chemical composition changes and their influence on water spectral pattern.
Viruses isolated from a portion of the blood sample that was used for CD4 count determination were used for the neutralization studies described below.
Twenty-seven patients did not have a CD4 cell count determination made after 24 weeks for the following reasons: 17 patients either died, were lost to follow-up or transferred to another institution and 10 patients either did not have CD4 counts ordered by the physician or there was insufficient blood volume.
While the instrumentation required might seem prohibitively costly for routine use in many areas of the world where the incidence of tuberculosis is high, if the HIV infection rates are also high, flow cytometry may have been previously established for CD4 count determination; the province of KwaZulu-Natal in South Africa is one example of this.
For glucose and blood count determination, blood was drawn into S-Monovette® tubes with K3-EDTA.
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