A more thorough way to identify functional TA genes, instead of in silico screening, was done via shotgun cloning which had revealed that many toxin genes could only be cloned when the neighbouring antitoxin genes were present (Sberro et al., 2013).
For many of the clusters in this set, there were no other clones in our dataset that mapped to the same cluster, so in many cases we could only compare a clone to a subset of the clusters to which it belongs.
BRCA1 antisense subclones demonstrated other traits associated with a tumorigenic phenotype, such as the ability to grow in soft agar independent of estrogen, whereas control clones could only form colonies with the addition of estrogen.
While the fellow being interviewed looked exactly like Mr. Crowe, we could only assume it was a clone, for this Mr. Crowe was charming, amusing, self-deprecating, and generous in his tips to young actors.
Sequence-specific interference with VHSV could only be observed with EPC clones transformed with a mixture of the three shDNAs, rather than with those clones obtained with individual sh DNAs.
Thus far, we could only retrieve Xrra1 containing genomic clones from mouse (Mus musculus, Mm) (GenBank accession no. NW_000328) and rat (Rattus norvegicus, Rn) (GenBank accession no. AC127923).
During the second round of screen, we found that this phenotype was somatic-dependent, as mislocalization of the oocyte could only be observed when large clones of homozygous mutant follicle cells were induced.
However, only six copies were found on the database given that two cloned copies (AIS76195 and AIS76200) shared the same amino acid sequence identity of 99.7 % with one copy of the database (BnaA05g31780D), and one cloned copy (AIS76201) could only share its highest identity (97.6%%) with BnaAnng02420D, which already shared 100%% of identity with AIS76196.
At relapse, the original TP53 mutations in all three TP53-mutated patients studied were retained, but the mutant level in one of them was much reduced at the time of AML relapse, as it could only be detected by a sensitive cloning technique but not by direct sequencing (patient 30, Table 5).
