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Nevertheless, rigid sedimentation, which could not be reverted to a uniform suspension by agitation, was engendered in PCLSs for α ≥ 70%%.
Interestingly, the presence of the protein synthesis inhibitor ciycloheximide produced an almost complete suppression of RXR-γ steady state mRNA levels (Fig. 3A), which could not be reverted by the addition of BPE and forskolin.
When VEGF-A165 was present at 10-fold excess over the VEGF-binding proteins, loss of claudin-1 could not be reverted indicating an insufficient inhibition.
Initial experiments of expression in E. coli BL21 showed that the streptavidin chimeras were only found in the insoluble fraction, which could not be reverted by modification of the temperature, IPTG concentration, or induction time of the cultures.
Interestingly, Rac1-mediated perturbation of SG assembly could be reversed by constitutively-active RhoA (V14RhoA), whereas, impairment of SG assembly caused by expression of dominant-negative RhoA (N19RhoA) could not be reverted by dominant-negative Rac1 (Fig. 2A lower panel, B).
Similar(55)
Alternatively, X3x could also be a mutation that occurred in the forward trajectory and increased phosphotriesterase activity, in which case it could also not be reverted.
Results with doxorubicin could not be determined on the highly resistant MCF7dox cell line since that chemotherapeutic agent had to be applied in such high doses that the measurements were disturbed by its own color (data not shown); paclitaxel resistance of this cell line could also not be reverted.
Once it is cheese, it can not be reverted.
For new columns, regeneration by TFA treatment had a drastic effect on column performance that could not be fully reverted even by very long reduction and rinsing periods.
The last isolate, which was obtained 3 years after miltefosine withdrawal and could not be subcultured, had reverted to the wild-type allele (L832).
But his Labour counterpart Stephen Twigg said education could not be improved by reverting to a system that was considered out of date 30 years ago.
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