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Because of the high polymorphic rate, the C. savignyi genome could not be assembled using the classical whole-genome assembly method.
Due to their highly repetitive nature, transcription activator-like (TAL) effector sequences present in strains CFBP 5825R and CFBP 1606R could not be assembled and are not represented in the final assemblies.
In all instances the loci were present in the short-read sequence data, however read depth was low (2-5×) and therefore could not be assembled by the parameters set for the Velvet assembly.> -wrap-foot> § For each CDS that had either a failed assembly or paralogous cross-identification error the entire CDS length was counted as affected.
Less abundant or lowly expressed transcripts could not be assembled into larger contigs resulting in singletons in the assemblies, termed singleton ESTs or singleton ETs.
This suggests that there were numerous viruses present in these viromes that could not be assembled due to a relatively low abundance or limitations in the assembly process.
The sequencing produced >12 million reads of 33 bp, which could not be assembled into a reasonable number of contigs using widely used de-novo assemblers (Methods S1; Table S1; Figure S1).
Similar(12)
But the sleek frame couldn't be assembled by welding parts together carbon doesn't melt when heated, it just chars.
Our genome coverage was approximately 76%, which, given some of the scaffolds may be overlapping and could not be properly assembled, might be slightly overestimated.
Genomic regions containing nonribosomal peptide synthetase genes or IS elements could not be properly assembled because of their highly repeated sequence patterns.
While the whole-genome sequence (WGS) data provided valuable information concerning strain diversity, the stx-converting phage genomes, critical for understanding toxin production, could not be fully assembled.
Unassigned BACs, also referred to as chromosome 0 BACs, lack sequence alignment and mapped markers so they could not be reliably assembled into any of the pseudomolecules.
More suggestions(15)
could not be ascertained
could not be mobilized
could not be arranged
could not be filed
could not be devised
could not be sampled
could not be relocated
could not be gleaned
could not be incorporated
could not be coalesced
could not be laid
could not be summarized
could not be made
could not be photographed
could not be tempted
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