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To realize well-controlling supramolecular assembly, a core-expanded naphthalene diimides derivative (1) was designed and synthesized as an n-type organic semiconductor and also as a halogen bonding (XB) donor that could form complementary XBs with 2,2-dipyridine or 2,2-bipyrimidine acceptor.
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Given structural constraints, such as the ability to form complementary base pairs and a linear covalent polymer, a variety of structural isomers of RNA could potentially function as genetic platforms.
Why would the proto-adapters form complementary RNA complexes?
This detection was rendered possible by the highly complementary 5′- and 3′-sequences that viral ssRNAs contain because these highly complementary sequences could form short double-stranded structures with perfectly blunt ends.
The results showed that only a complementary sequence could form a dsDNA with the Cu-DNCs DNA probe and give an obvious electrochemical signal.
The hybridization events were evaluated by ECL measurements and only the complementary sequence could form a double-stranded DNA (dsDNA) with DNA probe and give strong ECL signals.
This strategy also avoids inadvertent cleavage of adapter sequences, as there is no complementary strand with which they could form dsDNA. DSN operates efficiently at higher temperatures and requires at least ten perfectly complementary base pairs to cut, thus it has better mismatch discrimination than RNase H, especially given the higher Tm of RNA DNA compared to DNA DNA hybrids.
Secondary structure analysis revealed that the ∼80 bp before and after the unsequenced region are highly complementary (∼80%) and could form a secondary loop.
Despite the strong conservation there were 50 stem 1 changes, allowing us to determine whether fully complementary double-stranded sequences could form due to existing reciprocal mutations either within the same unit, or in another unit of the same species.
Shape complementary suggests that a tighter complex could form than the one that is visualized in this crystal structure.
Especially, the 5′ and 3′ ends of the 17S rRNA have complementary sequences (Fig. 4F), and could form a long helix (Young and Steitz, 1978).
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