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In our attempts to detect small numbers of cells, we initially used a serial dilution method and could detect down to 3 cells in vivo.
The assay could detect down to 0.001 TCID50/ml with corresponding Ct value of 34.7.
Previous studies showed that soluble hCG receptor (sLHCGR) in combination with PAPP-A could detect Down's pregnancies which remained undetected by PAPP-A plus free- βhCG.
The sensor could detect down to 10 attomolar target DNA.
The assay allowed differentiation of Pba from other Pectobacterium and Dickeya species and could detect down to 10 cells mL−1 bacteria.
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The extraction control qPCR could reliably detect down to 37 Ct which is approximately equivalent to 1 pg of human genomic DNA or one-third of a human genome or 88 copies of the target 18S rRNA gene.
Five independent mixing studies using mutation-discordant pairs of cell lines and patient specimens demonstrated that the COLD-PCR enhanced melting curve assay could consistently detect down to 1% mutant DNA in a wild type background.
The Aspergillus qPCR could reliably detect down to a threshold cycle (Ct) of 41 which is approximately equivalent to 1 fg of Aspergillus genomic DNA or a single copy of the target 18S rRNA gene.
Using human vascular endothelial growth factor as a model protein, the designed aptasensor could detect protein down to 0.82 pg mL−1 with a linear range from 1 pg mL−1 to 1 ng mL−1.
The 95-D cell line containing 2.8 × 10 mannose units per cell could be detected down to 580 cells/mL, while the H1299 cell line having 3.1 × 10 mannose units per cell could be detected with unprecedented LOD of 12 cells/mL.
Moreover, the p53 gene could be detected down to 1 nM with a linear response range of 1×10−9 3×10−7 M, and p53 gene point mutation was readily distinguished from the wild-type one.
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