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All LCM-captured samples yielded sufficient total RNA (10 – 20 μg) that could be quantitated by the low-range Ribogreen RNA Quantitation Kit (Molecular Probes).
The percentage of chimerism could be quantitated either by agarose gel analysis or Southern blot analysis.
Each of the metal complexes can be easily identified by their characteristic retention times and could be quantitated using estimated molar absorption coefficients.
Incubation of THP-1 cells at 37 °C with fluorescence-labeled platelets and anti-platelet antibody promoted ingestion of platelets that could be quantitated by flow cytometry.
With this method, 10 10 DDC-expressing gastric cancer SNU-16 cells per 10 mesothelial cells could be quantitated.
Four different environmental extracts were examined on each method as verification that OPAHs could be quantitated successfully in environmental matrices.
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Thus, live cells could rapidly be quantitated and distinguished from dead cells in a population without the need to determine CFUs on agar plates.
Due to the high concentration in the supernatant the internal fluorescein concentration could not be quantitated during in-diffusion.
The ratio was not determined when the target gene could not be quantitated in both the paired samples.
However, due to the limited amount of RNA available for the cancers, these preliminary observations could not be quantitated.
Additionally, the general COL11A1 transcript could also be quantitated in a novel assay (e.g. multiplexed with A and/or E variants) in order to identify samples that although they are positive for A and/or E variants don't sum up to the total COL11A1 transcript and therefore one could hypothesize that they contain additional aberrant transcripts.
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CEO of Professional Science Editing for Scientists @ prosciediting.com