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Most of the genomes constituted, on average, 1% (min. 0.25%, max. 5.96%) of the total reads in their respective metagenomes; in total 10 14% of the metagenome reads could be mapped back to genome bins (Supplementary Table 5).
The learned dictionary comprised atomic spectra, which could be mapped back to pitches.
Once identified, these task characteristics were specifically defined and used by test developers to create items that could be mapped back to the framework.
With the current enrichment, out of all reads that could be mapped back to the human genome, satellites comprise 4.5%, LINEs 10.5%, and SINEs 11.4% (in the genome satellites comprise 0.45%, LINEs - 21.1% and SINEs – 13.5%).
This approach permitted the automatic analysis and view of cortical surface and a significant (threshold of p<0.05) t-test contrast between TNP and HC groups, which could be mapped back from the average brain to each individual brain (Figure 1).
Of the single reads, 84% could be mapped back onto scaffolds.
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This also suggested the presence of highly divergent sequences among the strains, from which the reads could not be mapped back to the reference T. parva Muguga sequence but could be assembled de novo.
Hairpins that could not be mapped back to the genome were regarded as false positive candidates and were not considered.
The reads could then be mapped back to contigs to obtain longer sequences using paired-end reads as a guide.
After further investigation, more than 85% of the unknown category (data not shown) in the 2 libraries could not be mapped back to the human genome (hg19).
The number of unmapped reads observed in the Mondrian samples exceeded 1%% in the 5 and 1 pg libraries, while all MALBAC libraries contained high levels of reads that could not be mapped back to the reference genomes, suggestive of significant biases associated with the MALBAC amplification procedure.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com