In total, 646 differentially expressed array probes were identified (see Additional file 2), which could be further divided into 265 probes (206 genes) with higher expression in DoxHPC1 and 381 probes (295 genes) with higher expression in DoxHPC7.
Of these, 10,460 probes could be further grouped into 3529 regions of 2 or more DM probes within 1 kb, which we designated DMRs; (Supplementary file 5); the numbers of probes and regions identified as DM at a range of mean interspecies β thresholds are given in Supplementary file 6.
We note that probe hybridization rates could be further increased using peptide nucleic acid (PNA) probes, because the neutral charge of the PNA backbone is expected to reduce the electrostatic repulsion between the probe and the target (Kuhn et al., 2002).
We believe that these probes could be further developed for early disease detection, as well as prognostic markers, of colorectal cancers.
Such refined alignments at the superfamily level can be found rarely and could be further used as reliable evolutionary models to probe various issues of conservation, length variation, structural and functional aspects of protein universe.
We identified a signature of 219 probes that defined the KC PTEN phenotype and could be further refined to as few as eight probes.
The data could be further divided into individual amplicon-specific probes.
This work could be further developed by implementing the RNA probe design, library dimension, and total target size.
Despite the fact that IOPTH and intraoperative frozen sectioning has a 95% success rate [ 5, 7, 8] for determining surgical cure this could be further improved with use of a radioguided probe [ 41] and significantly reduced time in the OR.
There is a possibility that selectivity could be further increased after optimization of immobilized oligonucleotide length, as well as the density of probes on the transducer.
