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In addition, further research is needed to develop cost effective sequencing study designs and analysis methods that incorporate the uncertainty in the reference haplotypes and imputation into the association analysis.
Next generation sequencing (NGS) collectively describes these recently-developed genetic technologies that enable rapid and cost effective sequencing of large amounts of DNA.
Additionally, improvements in exonic enrichment protocols will usher in an era of cost effective sequencing of all the amino acid coding bases of genomes.
They are produced by an automated and cost effective sequencing mechanism that, however, results in low quality sequences which typically do not cover the complete transcript.
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Although the read throughput of the Ion PGM is lower than that of GAIIx and Hiseq sequencers, the cost-effective sequencing and the rapid runtime (4.5 hours per run) of this platform facilitates routine analysis.
We present the design and analysis pipeline of rare-variant studies and review cost-effective sequencing designs and genotyping platforms.
We present a strategy that combines cutting edge next generation sequencing with "old school" genomics resources and allows rapid cost-effective sequencing of plant genomes.
Therefore, the most cost-effective sequencing strategy depends on the particular organism.
The recent availability of new sequencing technologies provides the capability to a rapid and cost-effective sequencing of small genomes.
Cost-effective sequencing methods and tools have surpassed manual annotation as the amount of input data has increased by orders of magnitude.
In spite of recent progress in the development of faster, more cost-effective sequencing technologies than used in the first genome projects [8], [9], it is still unrealistic to sequence all species of interest de novo.
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