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However, the ephrinA2 and EphA5 were up-regulated only in selected layers in the cortex of the mutant; expression levels in other layers did not change.
The entire cortex of the mutant was filled with disorganized, haphazardly positioned cells (Fig. 2B).
The adjacent gray matter and the cortex of the mutant mice were almost entirely spared (Additional file 2: Figure S1).
Since there were significantly fewer FoxP2-labeled neurons, and because the macrocephaly occurred post-natally, we asked whether the total number of NeuN-positive neurons or S100-positive glia in the cerebral cortex of the mutant animals were different.
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Differences in the size of the area activated by whisker stimulation may therefore provide evidence of altered transcolumnar connectivity in the disturbed cortex of the reeler mutant.
For the study of Ngn2 targets, data from the cortex of Mash1-/; Ngn2-/ double mutant mice was utilized rather than Ngn2-/ single mutants in order to avoid the compensation due to de-repression of Mash1 in the cortex of the later mutants [ 2].
Our data demonstrate that the number of CGE-derived cells is not altered in the cortex of the double mutants.
This result excludes the possibility that the more severe reduction of the MGE-derived cells in the cortex of the double-mutant mice is due to a further decrease in cell cycle exit.
First, we found that the brain of the Nde1 Lis1 double heterozygous (Nde1+/−Lis1+/−) mutant was about 20 25% smaller than that of Nde1+/− or Lis1+/− mice (Fig. 1A), and the cerebral cortex of the Nde1+/−Lis1+/− mutant showed preferential thinning of cortical layers II/III similar to what was observed in the Nde1−/− mutant (Fig. 1B and C).
Quantitation of both cell populations did not reveal a significant difference between the total number of NeuN-positive neurons (Fig. 5C) or of S100-positive glia (Fig. 5H) in the cortex of the control versus mutant animals.
In this regard, downregulation of another apical protein, δ-catenin, has been described using a transcriptome analysis of the cortex of Pax6 mutant mouse (Duparc et al., 2006).
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