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Thus, ISH analysis corroborated the changes in c-myc expression levels that were previously determined by qPCR assays.
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In order to corroborate the changes in gene expression identified by the oligonucleotide microarrays, we used a real-time (kinetic) quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) approach, as previously described (Naciff et al. 2002).
To corroborate the changes in the oxidative burst in the NILs in response to D. noxia biotype SA1 feeding, leaves were collected and stained for H2O2 using 3,3′-diaminobenzidine (DAB), which forms reddish-brown polymerized deposits in the presence of peroxidase (Fig. 6).
This hypothesis would fit with the rapid development of hepatic steatosis corroborating the change in feeding habits and hence of intestinal microbiota.
This self-assessed increase in knowledge corroborated the changes on the content test administered at both the beginning and end of the BrainU 101 workshop.
These results corroborate the changes engineered in the recombinant CRP subunit and verifying the expressed protein is in other ways identical to the biologically derived CRP molecule.
We further corroborated these changes in gene expression during fruit ripening at gene-level and validated the microarray results by qRT-PCR for four selected individual genes that appeared enriched in these categories.
These flow cytometry analyses corroborate the observed changes in MRSA cell shape seen in TEM images following exposure to the leachates, demonstrating that the appearance of distorted cells is characteristic of leachate exposure and not due to TEM processing.
Experimentally induced dissociations between firing rate and gLFP and the associated changes in fMRI BOLD signal or regional cerebral blood flow have corroborated the view that changes in regional cerebral blood flow or BOLD are strongly linked to gLFP, not to the local firing rate (Mathiesen et al., 2000; Rauch et al., 2008).
Moreover, they elegantly use AFM to corroborate that changes in their pathway lead to predictable alterations in tension of the actin rich cortex in the Neuro2A cell system.
Microscopy results were corroborated by inspecting the change in PML protein level by conventional immunoblot analyses under the above-mentioned conditions of CK2 activation and inhibition.
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