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Quantitative Real-Time PCR experiments were carried out to corroborate the expression patterns of a subset of sequences (i. e. 89 out of 642 unisequences, equal to 14%), corresponding to 61 different genes.
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An explanation for the weak activation of downstream pathways by medaka Egf and Areg may indicate a better reactivity of medaka Egfra with medaka Hbegf when compared to other ligands, corroborating the expression patterns in adult organs data where medaka egfra was found to be preferentially expressed together with hbegf.
Interestingly, the early over-expression of DQ198153 transcript in the Moro is corroborated by the expression patterns inferred when considering the EST-based tissue/organ distribution, as reported in the section ' In silico identification and characterization of C. sinensis GST transcripts'.
Several online reference atlases of mRNA expression were used to corroborate the expression of genes identified in the microarrays.
To corroborate this model we studied the expression patterns of lat-1 and ten-1 using transcriptional reporter transgenes.
We selected adhesion genes involved in cell-to-matrix and cell-to-cell interactions to corroborate by qPCR the expression pattern inferred from Illumina sequencing.
This is corroborated by similarities observed between the expression patterns of native LigA and the fluorescence levels produced by the PAG reporter strain, in comparison to the non-induced controls (Table 2).
QPCR was performed on NRG1 to corroborate this expression pattern.
See main text for details of the expression patterns.
However, the expression patterns were different.
Results for all these transcripts corroborated the expression differences observed in our SAGE analysis.
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