While crystallization in small volume droplets is usually characterized by a low nucleation probability and correspondingly low number of crystals, we did not observe this in our experiments.
Surprisingly, in the four cell lines with modest reductions of PSMD2, we did not detect constitutive activation of NRF1, and correspondingly, the expression of 20S subunits was unaltered.
We did not observe increased apoptosis over the different time points between E10.5 and E12.5, and correspondingly neural crest contribution to the outflow tract was not significantly reduced at E11.5 (see Supplementary material online, Figure S3B D ).
Again, we did not see evidence for additional impairment of cell growth: all agents increased the total protein/DNA ratio significantly, with a correspondingly greater effect at 8 days, when cell number was reduced the most.
Consequently, we do not preclude the possibility that enabling a correspondingly more detailed chemotaxis mechanism might further improve cell aggregation characteristics, and nullify the observed differences in outcome.
To reiterate, we do not imply that MCS is 'better' or 'worse' than other methods, but we do think that the evidence shows that it is different and correspondingly valuable, and should thus be used in parallel with fingerprinting methods, whether separately or (as often done to advantage, e.g. [63, 143, 144]), via fusion methods.
Correspondingly we found T1R2 did not colocalise with CCK, whereas 32% of FFAR3-positive cells were CCK-IR.
It is too easy to blame the faded sentimentality of his worst stuff on the Georgians, to whom he attached himself through the early influence of Eddie Marsh and Edmund Gosse (correspondingly they did not much care for the raw tone of his war poetry).
Correspondingly, hypoxia did not cause major changes in the morphology or ultrastructure of surviving islet cells as examined by electron microscopy (ESM Fig. 3c).
