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Individual HCV peptide (final concentration 4 μg/mL) was then added to corresponding wells.
Twenty-one days after reprogramming, ten colonies of iPSCs generated from the MEFs harboring the GFP reporter gene on an inactive X chromosome were picked up at random, and each colony was divided in half and passaged into corresponding wells in two separate plates (Fig. 5a).
The upcoming phase II project will be carried out in 2013 which will cover the construction of 2-3 corresponding wells nearby the first well some 50 m away in relevant azimuth, coupled with a sophisticate measurement and monitoring program to collect the baseline data, prior to the injection test operation.
A total of 49 attribute maps were generated for all the fields and superimposed on their corresponding wells.
The present study is based on Vertical Electrical Sounding (VES) associated to water boreholes (lithological columns and corresponding wells logs).
Microorganism suspensions at 106 CFU/mL (Colony Forming Unit/mL) concentrations were inoculated to the corresponding wells.
It can be speculated that the more high-yield mutants dominate in the mixture, the higher pigment production could be observed from the corresponding wells.
Nine percent (v/v) precultures were inoculated in the corresponding wells of new 48-deep MTPs containing 1.0 mL fermentation medium.
Using a microarray scanner, the glass slide can be interrogated for antibodies of interest that were secreted by the cells in the corresponding wells.
The vitrinite reflectance values were posted on the isopach map against the corresponding wells where oil samples were obtained from the well head.
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Transfer 4µL of amplified PCR product to its corresponding well.
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