A similar disconnect is seen with component of the basal transcriptional machinery, TBP and TFIIE, which are broadly recruited to Plasmodium genes regardless of corresponding transcript abundance [ 9].
Overexpression of the VoFPS cDNA increased levels of the corresponding transcript 4- to 8-fold and sesquiterpene hydrocarbon accumulation by 1.5- to 4-fold.
Overexpression of the VoVDS cDNA increased the corresponding transcript levels 5- to 9-fold and markedly increased yields of the oxygenated sesquiterpenoids valerenic acid and valerenal.
The ACTIN-2 control probe detected its corresponding transcript in both wild type and rlk902.
This approach also detected proteins whose corresponding transcript expression was not detected by microarrays.
Viral replication of each mutant FMDV was analysed by transfection of the corresponding transcript into BHK-21 cells.
The majority of proteins identified by LC-MS/MS had detectable levels of their corresponding transcript by microarray.
Probes were designed to hybridize between exons to ensure the detection of the corresponding transcript avoiding genomic DNA amplification.
As expected, CpG sites at which methylation was positively correlated with expression of the corresponding transcript were rare.
However, the low level of cDNA amplification observed for MSP3.5 suggests lower stability of the corresponding transcript (Fig. 4, panel A, top right).
TaqMan® probes encompassing fragments of areas from the extremes of two exons were designed to ensure the detection of the corresponding transcript avoiding genomic DNA amplification.
