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Arenaviruses use RNA as their hereditary material; RT-PCR creates a DNA template corresponding to viral RNA genes.
For this purpose, several pairs of primer amplifying regions corresponding to viral G and N genes were assayed.
Current reverse genetics approaches for engineering the BTV genome rely upon in vitro synthesis of capped RNA transcripts from cloned cDNA corresponding to viral genome segments.
As I described above, RT-PCR creates DNA templates corresponding to viral RNA, copying them many, many times, so that even if there is only a small number of genes in a sample, they can be detected and isolated for study.
For example, inserting oligonucleotides corresponding to viral readthrough regions into the 5' end of the GUS reporter gene demonstrated that nucleotides located immediately 5' and 3' of the leaky stop codon act as critical determinants of readthrough efficiency [26], [28].
Percentage of hits corresponding to viral sequences.
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To incorporate the effects of the bacteriophage infection, a new subset of metabolites corresponding to the viral proteins and the viral genome was added to the uninfected model.
Viral supernatants were diluted in medium to obtain a multiplicity-of-infection yielding 10 20% GFP-positive cells corresponding to single viral infections.
From all of the above experiments we conclude that no transcripts corresponding to structural viral proteins were detected.
Both plasma and P. falciparum determinations were available for 176 (93%) of these children, 87 (49%) corresponding to the viral group and 89 (51%) to the invasive bacterial one.
A fast increase in intracellular viral RNA content was observed in the first hours post-infection (hpi), both in the presence or absence of AZT, most likely corresponding to incoming viral RNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com