Each panel from the top down shows the trend over an increasingly longer time span, with the shaded area corresponding to the time span for the panel directly above.
The model employed herein solves conservation equations for momentum, energy, and species on a one dimensional (1D) domain corresponding to the line spanning the domain between nozzle orifice centers.
In MALDI-TOF/MS analyses carried out, a mass-spectrum m/z 1910.8 or 1910.9 corresponding to the peptide spanning amino acid (aa) 356 377 (SSGSPYGGGYGSGGGSGGYGSR; see Fig. 3) with its Ser359 (Fig. S1) unphosphorylated was reproducibly detected, while a mass-spectrum m/z 1990.8 corresponding to the peptide with the Ser359 residue phosphorylated (Fig. S1), was always absent (Fig. 4A and B).
For each of the 9,041 genes contained in the PIN, we analyzed the genomic region corresponding to the transcript spanning the longest chromosome region.
We closed the gap corresponding to the membrane-spanning repeat region of tolA with Sanger sequencing, using Phred/Phrap/Consed [ 39- 41] to assemble and manually examine the sequence.
Reverse cross-linked immunoprecipitated chromatin was subjected to both RT-PCR and RT-qPCR using primers corresponding to the specific region (spanning the 10 putative Isl1 binding sites).
To this end, a 1/10 scale model of a double-layer lattice space structure corresponding to the prototype structure with span of 40.8 m was designed.
cDNA samples prepared from RNA extracted from immortalized cell lines were used to amplify by PCR three fragments corresponding to the ATR cDNA region spanning nucleotides 5748 5858 (NM_001184), the ATR splice transcript spanning nucleotides 5626 5857 and including a deletion of the last 121 nucleotides of exon 33, and a fragment spanning nucleotides 450 619 of 18S RNA.
Additionally, there is a molecule of 2-methyl-2,4-pentanediol in CysK corresponding to the region of CysM spanning the C-terminus of CysO and the PLP cofactor.
The genomes were searched using tBLASTn with the amino acid sequence corresponding to the region of Sinbad spanning from the Cys-His box to the conserved protease catalytic domain (bp 1588 2236) [see Additional file 1] as the query.
