The limit of quantification corresponding to the lowest concentration being determined with a specified relative standard deviation, commonly 10%, was considered as 3 times the limit of detection.
Time points were taken every 1.25, 2.5, or 5 min over a total time of 90 min. For quantification, Z-stacks corresponding to the shown time points were projected in single pictures as maximal Z projection (ImageJ); pictures were assembled in time-lapse stacks and the same positions in every slice were quantified as described.
Quantification of bands intensity corresponding to the Hpt precursor by Imagequant TL software validates the differential serum level observed by MAP.
Using quantitative PCR, we quantified the total number of viral copies by determining an absolute quantification of HBS DNA corresponding to the different types of HBV DNA in the tissues, that is, episomal, integrated or the covalently closed circular DNA (cccDNA).
Additional details of the protein quantification results for the proteins corresponding to the 21 genes are shown in Supplementary Table 1.
Values obtained by semiquantification of pendrin protein expressed as arbitrary units and quantification of pendrin transcript signals corresponding to the tumours and paired normal tissues are shown in Figure 2C.
Three bands, corresponding to the upper, middle and lower parts of the gel, were selected for quantification.
By using the color quantification q stated above, we define q 1 and q 2 corresponding to the J and K color components.
Sections corresponding to the aforementioned Bregma locations were imaged and a region of interest (ROI) randomly selected for quantification by an observer blind to genotype and treatment.
For miR-21 quantification, the following histologically stained structures were differentiated: blue areas (B) corresponding to the hybridization signal, red area (R) corresponding to the red nuclear stain, purple areas (P) corresponding to blue ISH signal overlaying nuclear red stain.
