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The levels of mRNA encoding for biglycan and decorin were evaluated in corresponding tissue samples by reverse transcriptase polymerase chain reaction (RT-PCR).
Mutations in exon 1 at codon 12 and 13 of the N-RAS gene were detected in four cell lines and three corresponding tissue samples, respectively.
This is supported by our observation that in five cases mutations in the N-RAS gene were present in tumor cell lines but not in the corresponding tissue samples.
Moreover, PrPSc was detected by serial DSP-PMCA in the palatine tonsils, lymph nodes, ileocecal region, and muscular tissues (Figure 6A), whereas no PrPSc signal was detected in the corresponding tissue samples from uninfected control cow 2914 (Table 2 and Figure 6B).
The probe sets with detectable expression signals in corresponding tissue samples were selected for classifier construction.
E. coli in the corresponding tissue samples was quantified by Real-Time PCR, as described previously [ 21].
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All of the B-RAF mutations in exon 15 were concordant in cell lines and corresponding tissues except in five cases: The cell line UKRV-Mel-29 but not the corresponding tissue sample carried the V600K mutation, whereas the corresponding tissue biopsies but not the cell lines UKRV-Mel-11, Ma-Mel-104, Ma-Mel-113 and Ma-Mel-121a carried the V600E mutation.
Swab samples were usually positive only when the corresponding tissue sample was strongly positive.
All of the genes found to be methylated in serum samples were also methylated in the corresponding tissue sample.
For each region, the expression patterns of contextual gene sets show strong correlation for the corresponding tissue type samples (see Additional file 4: Figure S2 B-E)).
Comprehensive transcriptome studies of cancers often rely on corresponding normal tissue samples to serve as a transcriptional reference.
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