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The corresponding staining to the primary hepatocyte served as the positive controls.
No corresponding staining was observed in any of the negative control samples.
The index was calculated by multiplying the percentage of tumor cells by the corresponding staining intensity, as described previously [ 33].
Half of the follicles were labelled at 2 months for Tg-I but the corresponding staining decreased at 6 and 10 months.
Furthermore, as the MCT1 staining profile in the illustrated CTCs does not match the corresponding staining intensity observed in the DAPI channel, this signal is not considered nuclear.
All DNA values were calculated in relation to a corresponding staining control, which obtained the value 2c, denoting the diploid DNA content.
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Each core was semiquantitatively scored on a scale of 1 4 corresponding to staining intensity and percentage of cells with positive nuclear staining: (1) low/negative staining <5% positivity; (2) focal/patchy staining 5 25% cell positivity; (3) moderate staining 25 50% positive and (4) strong staining >50% positivity.
Tumor disruption and cell death was apparent in hematoxylin and eosin (H&E -stained sections as a wH&E -stainedd central area (Fig. 7A) with corresections ISEL stasning (Fig. 7B).
In box: vital tumor area with highest radioactivity uptake and (b) corresponding H&E staining.
(A) Representative histograms and absolute MFI values of CXCR5, Bcl6, and PD-1 or corresponding isotype staining of CMFDA-labeled OT-II T cells at indicated times after OVA/alum/LPS immunization.
f, i, l represents corresponding DAPI staining.
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