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There is no corresponding secondary structure element in chain B, Fig. 6a.
Finally, the obtained satisfying solution is then distributed or returned to the corresponding secondary users.
The reduction of 1 to its corresponding secondary alcohol 6 was additionally shown.
The results demonstrated that both the supramolecular architectures and their corresponding secondary structure can be tuned just by terminal variations.
After electroblotting, nitrocellulose membranes were incubated with specific primary antibodies and the corresponding secondary antibodies.
A 1∶28,000 dilution of the corresponding secondary antibody was used.
The blots were then incubated with corresponding secondary antibodies conjugated to alkaline phosphatase.
The membranes were incubated with corresponding secondary antibodies and the signal was visualized by using a chemilluminescent kit (Pierce Bioscience).
Crf2 was separated by SDS-PAGE, followed by immunoblot using anti-Crf2 scFv and the corresponding secondary antibodies (Table 3).
The corresponding secondary antibody (DAKO Biotech, Inc. Glostrup, Denmark), labeled with FITC, was added at room temperature for 1 hr.
After being washed, slidses were incubated with the corresponding secondary antibody conjugated with HRP or AP for 1 h.
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