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(Note that the underlined sequences correspond to the added corresponding restriction sites).
The amplified ORF were restricted with HindIII and XbaI and cloned between the corresponding restriction sites of the mammalian expression vector pcDNA3.1.
*Restriction sites with corresponding restriction enzyme are represented in italics.
The amplified genes were inserted into the corresponding restriction site of pETDuet-1 (Novagen; Madison, Wl).
The amplified genes were inserted into the corresponding restriction site of pACYCDuet-1 (Novagen).
The sequences of the restriction sites are underlined and the corresponding restriction enzymes are annotated.
The second part will propose the model of the emergency task planning and specify the corresponding restriction conditions.
The agarase DNA fragment was excised from pJET1.2 by using KpnI and XhoI and was subsequently subcloned into pET-29a at the corresponding restriction sites.
The isolated product was digested with EcoRI and PstI enzymes, as above; corresponding restriction sites flanked the pUC18 multiple cloning site (MCS).
After digestion with the corresponding restriction endonucleases (NEB or Fermentas, Beijing, China), segment was ligated into pET-28a to construct the carrier plasmid pX12345LT.
The corresponding restriction for the allowed α values is: frac{{1 - eta_{text{CS}} }}{{1 + eta_{text{CS}} }} < alpha < frac{1}{{1 + eta_{text{CS}} }}.
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