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Further, fifteen out of the 24 cases, in which differential DNA methylation corresponded with differential gene expression, consisted of hypermethylated CpG sites and corresponding repression of the transcript.
Transfer of cells from glucose to glycerol led to a corresponding repression of ribosomal biogenesis genes (Fig. 2c).
In addition, miR-26a was verified to be involved in the negative regulation of TLR3 signaling by targeting TLR3 itself in macrophages, and modifications of miR-26a function exhibited corresponding repression or augmentation of TLR3 signaling.
Modifications of miR-26a function by transfection of miR-26a mimics and inhibitors exhibited corresponding repression and augmentation of TLR3 and its signaling downstream cytokine expressions in NR8383 cells.
MiR-26a mimics exhibited corresponding repression of TLR3 protein by 40%and25%5% compared with the NC or mock group, whereas miR-26a inhibitors increased TLR3 expression 1.6-fold compared with the NC or mock.
Treatment of cultured cells in vitro with demethylating agents, either alone or in combination with HDACi, has been shown to re-activate expression of tumour suppressor miRNAs, such as miRNA-124a and mir-127, causing the corresponding repression of their oncogenic targets (Saito et al, 2006).
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This observation suggests that the reduction of the amount of Chl a might be caused not only by the down-regulation at the transcriptional level seen at the initial response phase, but also by other mechanisms like repression of the corresponding enzyme activity [30] and regulation of Chl a degradation.
Many genes (n = 84) provided evidence of a repression corresponding to a fold change of greater than ten.
We have found that Cr VI) is a strong developmental toxicant that, at concentrations as low as 0.1 μM, causes 95% inhibition of cardiac differentiation of EBs, corresponding to the repression of cardiomyocyte-specific gene induction.
These modifications can either be associated with activation or repression of corresponding genes in different cell types [ 22].
As CRP is a key regulator for catabolite repression, the corresponding binding sites can be found in or near many promotors involved in carbohydrate catabolism.
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