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The corresponding primary breast tumours for BM11 and BM15 were also analyses these both had correspondingly high MIs (82 % and 74%% respectively).
However, Pinto et al. showed that expression of BCL6 protein was significantly down-regulated in metastatic lymph nodes than in the corresponding primary breast cancer [ 14].
The GALNT9 promoter was methylated in 3/5 brain metastases and not methylated in any of the corresponding primary breast tumours (Fig. 2b).
In the present study, we determined the gene hypermethylation profiles of normal tissues adjacent to invasive breast carcinomas and investigated whether these are associated with the gene hypermethylation profiles of the corresponding primary breast tumors.
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Futhermore, our results demonstrate a strong correlation between the promoter activity and expression levels of the corresponding genes in primary breast cancer samples.
Eleven pairs of formalin fixed paraffin embedded (FFPE) primary breast tumours corresponding to matched metastatic brain tumours were provided by BTNW tissue bank.
Biopsies from primary breast cancer and corresponding metastases from 58 patients were studied.
Archival paraffin-embedded tissue from 69 patients with primary breast tumours and corresponding lymph node (LN) metastases was obtained from Imperial College NHS Trust tissue bank.
Recently, Jensen et al. [31] assessed the PIK3CA mutation status in both primary breast tumors and corresponding metastases and showed a high discrepancy between primary tumors and metastases with either a gain or a loss of PIK3CA mutation in metastases.
Using a previously developed enzyme-linked immunosorbent assay (ELISA), the levels of the receptor for urokinase-type plasminogen activator (uPAR) were determined in cytosols and corresponding membrane pellets derived from 878 primary breast tumours.
Global miRNA expression profiling was performed on 47 tumor samples from 14 patients with paired samples from primary breast tumors and corresponding lymph node and distant metastases using LNA-enhanced miRNA microarrays.
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