Because all the mutations were not detected in corresponding normal tissues, these mutations were confirmed as somatic mutations.
As described in Fig. 1A, among the total fresh samples, 10 HCC samples exhibited higher GNA13 expression compared to that in the corresponding normal tissues.
Real-time PCR results showed a much higher miR-21 expression level in the tumor tissues than in the corresponding normal tissues, whereas the BMP-6 expression level was higher in normal tissues.
Carbonic anhydrase IX (CAIX, gene G250/MN-encoded transmembrane protein) is highly expressed in various human epithelial tumors such as renal clear cell carcinoma (RCC), but absent from the corresponding normal tissues.
Overall, the metabolic gene expression program in tumors is similar to that in the corresponding normal tissues.
The significant high levels of FXYD6 were detected in tumors of liver, thyroid, prostate and colon, compared with the corresponding normal tissues (Fig. 1F).
We previously described its overexpression in colorectal adenocarcinomas compared to the corresponding normal tissues [10].
PCR amplifications were performed on genomic DNA templates from both tumor and corresponding normal tissues.
Taken together, there was a clear correlation of ALDH1 expression between tumors and corresponding normal tissues.
For Ep-ICD, the number of tissues analyzed were 38 breast cancers and 25 corresponding normal tissues, 49 prostate cancers and 9 corresponding normal tissues and 21 benign prostate hyperplasias, 57 HNSCCs and 20 corresponding normal tissues, 46 ESCCs and 20 corresponding normal tissues, 59 each of lung and colon cancers, 10 each of bladder, ovarian and pancreatic cancers, and 9 liver cancers.
Hence, these data suggest that not all tumours display relative hypomethylation globally compared to their corresponding normal tissues.
