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Raw fluorescence data were normalized with respect to the GAPDH expression level in all samples and the CLU transcript variants expression obtained in thyroid tumour samples was normalized to the corresponding normal samples.> -wrap-foot> - : data unknown.
Normalized expression data was extracted for the PLK1 probe set (202240_at), and the relative fold change to the geometric mean of the corresponding normal samples for each study was computed.
48 variants were also found in corresponding normal samples (Supplemental Table S4).
* The values represent the changes compared to corresponding normal samples.
from the mean of the corresponding normal samples were scored as showing AI or AEI.
The thyroid cancer and corresponding normal samples on the microarray were not paired well.
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Quantification of the hybridisation signal for the paired samples revealed that NDRG2 expression was reduced in 9 out of 10 colon tumor samples compared to the corresponding normal sample.
A twofold increase or decrease in the copy number of H2AX in tumor samples in comparison to the corresponding normal sample within the pair was considered as amplification or deletion, respectively.
MALDITOF was used to determine allelic imbalance at the XRCC1 Arg399Glocioci by dividing the allele frequency ratio of the tumor sample by the allele frequency ratio of the corresponding normal sample[ 26, 27].
Loss of heterozygosity was assigned to a cancer sample when the absorbance ratio (405 nm/620 nm) differed from the absorbance ratio for the corresponding normal sample by more than two standard deviations.
Because a corresponding normal sample of the MRT-8 case with CNTNAP2 R157C mutation was not available, we could not exclude the possibility that this mutation would be a rare SNP.
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