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Many of the actin-associated proteins that are reported to be upregulated in carcinomas, for example ezrin, are also expressed in the corresponding normal epithelium [2], raising uncertainty over their practicality as possible therapeutic targets.
Next, NGF (or TrkA, p75NTR) expression in each specimen was evaluated by computing the ratio of NGF (or TrkA, p75NTR) band intensity in tumour to that in the corresponding normal epithelium (T/N ratio).
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Finally, we compared ALDH activity in ovarian cancer cells and their corresponding normal epitheliums, ovarian surface epithelial cells.
For example, in those cancer types where ALDH1 was highly expressed in the corresponding normal epitheliums, such as liver and pancreatic cancers, ALDH1 was expressed in most tumor specimens at remarkably high levels.
Thus, we chose six types (breast, n = 69; lung, n = 66; ovarian, n = 65; colon, n = 67; liver, n = 67; and pancreatic cancer, n = 19) of epithelial cancers whose corresponding normal epitheliums were representative of each subtype.
We here review the structure/polymorphism, mRNA and protein expression profile of HLA-G in RCC and corresponding normal kidney epithelium, its mode of regulation and its functional consequences on immune responses.
The matched tumour/normal samples of invasive ductal breast carcinomas and corresponding normal breast epithelium (n=10) analysed in this study have recently been described (Veeck et al, 2006).
Total RNA was isolated from matched ESCC and corresponding normal esophageal epithelium tissues (n = 3) with TRIzol (Invitrogen) according to the manufacturer's instructions.
For DNA microarray, 21 pairs of gastric cancer tissue and corresponding gastric normal epithelium were obtained from a total of 21 patients who underwent gastrectomy at Iwate Medical University.
Surgically resected squamous cell cervical cancer biopsies and the corresponding carcinoma adjacent histo-phenotypical normal epithelium were obtained from 34 patients with informed consent.
The corresponding histology in Figure 4 (A) demonstrates normal epithelium, while Figure 4 (B) indicates mild dysplasia and Figure 4 (C) indicates squamous carcinoma.
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