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Cytoplasmic extracts and corresponding medium supernatants were analysed for amino acid composition by gas chromatography.
CM from (a,e) vehicle, (c,g) LPS treated (10 µg/ml) and/or diclofenac-pretreated (diclo 10 µM) A549 cells or corresponding medium (Veh) was added (indicated by arrow).
CM from ATI-like cells treated with (a f) vehicle, (c,diclofenacnac (diclo 10 µM), NS398 (1 µM) and (g,h) LPS (10 µg/ml) or corresponding medium (Veh) was added (indicated by arrows).
Cell sample preparation Culture receptor-overexpressed cells in corresponding medium under certain conditions.
N k denotes the complex refractive index of the corresponding medium.
Samples were immersed in 0.5 ml of the corresponding medium and this medium was renewed at every measurement.
Similar(27)
Stimulated samples were normalized to the corresponding medium-only control.
Subsequently, cells were washed with their corresponding labeling medium and then used to inoculate 500 ml of labeling medium.
Medium was removed 24 h after plating, the cells washed once with PBS and incubated with the corresponding conditioned medium (100 µl/well) for 24 96 h.
For O4 staining, cells were stained live in vitro: O4 was incubated 1 h (1 400) at 37 °C in the corresponding culture medium (OPC proliferation or OL differentiation) before PFA fixation and further antibody staining.
After 5 weeks in biotic environment, the films had fragmented to fine powder, while the films in corresponding abiotic medium still looked intact.
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CEO of Professional Science Editing for Scientists @ prosciediting.com