Notably, the numerical approach limited the need for extensive prototyping, thus reducing the corresponding machinery costs and time-to-market.
The corresponding machinery is nuclear encoded, and recognizes short stretches of sequence immediately upstream of the C to be converted [ 6].
On the other hand, the corresponding machinery of L. kluyveri has a much flatter response curve, in other words, it can tolerate sequences that deviate significantly from its peak response, albeit with reduced efficiency.
However, the interaction among the proteins in their corresponding machineries appears to be different [ 7].
To this end, we defined the main processes included in the gene expression pathway, listed the known components of the corresponding machineries in D. melanogaster (16 machineries comprising 664 proteins), and identified their orthologs in Ch. tentans as follows.
While these analyses were meant to illuminate the evolution of editing sites, they do not necessarily shed any light on the evolution of the corresponding editing machinery.
Overall, the emerging picture is that dinoflagellates are not only distinct in terms of genome size, content, and transcriptional regulation, but also rival the complexity of multicellular eukaryotes as evidenced by the presence of a rich set of smRNAs and the corresponding protein machinery.
After application of stringent criteria, we identified a set of 21 distinct and previously unidentified bona fide miRNAs and siRNAs alongside the corresponding core protein machinery for smRNA processing.
While we are unable to find a dif sequence corresponding to the XerS machinery, xerS gene in this species is located close to the GC skew shift-point (xerC: 1031814-bp, xerD: 1055574-bp, xerS: 1228715-bp, and GC skew shift-point: 1225733-bp), which is indicative of its functionality as shown in previous works [ 25, 26, 46].
Wolfe et al. [45] reported that the cp genome of Epifagus virginiana, a plant with non-photosynthetic chloroplasts, encodes only 25 proteins because the photosynthetic machinery and the corresponding genes are not needed.
Thus, it seems that the OmpF-EBV IL-10 preprotein is more compatible with the Sec translocase machinery than the corresponding HCMV IL-10 molecule.
