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Total and specific IgE levels were measured with UniCAP100 and corresponding kits (Phadia) using either commercial ImmunoCAPs for tomato (f25), potato (f35), hymenoptera venoms (i1 and i3), HRP (o400), and MUXF (o214), or the in-house-made ImmunoCAPs.
Measurements were done according to the corresponding kits instructions using a colorimetric autoanalyzer [ 30].
Unfortunately, simultaneous staining for TUNEL and protein aggregation (ProteoStat®) was not possible due to the overlapping emission spectrum of the dyes in the corresponding kits.
Microarray analyses, RNA amplification, labeling, hybridization and detection were performed following the protocols supplied by Applied Biosystems using the corresponding kits (Applied Biosystems).
Topo I and Topo II activities were determined with corresponding kits according to the manufacturer's protocol (TopoGEN, Port Orange, FL, USA).
Measurements of enzymatic activities of total antioxidant capacity (T-AOC), SOD, and malondialdehyde (MDA) contents were determined spectrophotometrically using the corresponding kits (Nanjin Jiancheng Bioengineering Institute, Nanjin, China) and following the manufacturer's protocol.
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Although different versions of the kit can be used with a GC with different detector types (FID, NPD, MS) or with liquid chromatography (LC-MS), our experience with the cheapest system (GC-FID), using the corresponding kit, which is the fastest within this family of kits (7 min run time versus 12 min with LC-MS), was very satisfactory, even with manual injection.
Locate the make and model of the speaker on the magnet and find the corresponding kit at online vendor or at a specialty shop.
The supernatant (50 μL) was used to detect catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) activity by using the corresponding assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
dEpitope refers to the epitopes of betatrophin antibodies in corresponding ELISA kits.
All these corresponding commercial kits were purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China.
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