Although different versions of the kit can be used with a GC with different detector types (FID, NPD, MS) or with liquid chromatography (LC-MS), our experience with the cheapest system (GC-FID), using the corresponding kit, which is the fastest within this family of kits (7 min run time versus 12 min with LC-MS), was very satisfactory, even with manual injection.
Locate the make and model of the speaker on the magnet and find the corresponding kit at online vendor or at a specialty shop.
Total and specific IgE levels were measured with UniCAP100 and corresponding kits (Phadia) using either commercial ImmunoCAPs for tomato (f25), potato (f35), hymenoptera venoms (i1 and i3), HRP (o400), and MUXF (o214), or the in-house-made ImmunoCAPs.
Measurements were done according to the corresponding kits instructions using a colorimetric autoanalyzer [ 30].
Unfortunately, simultaneous staining for TUNEL and protein aggregation (ProteoStat®) was not possible due to the overlapping emission spectrum of the dyes in the corresponding kits.
Topo I and Topo II activities were determined with corresponding kits according to the manufacturer's protocol (TopoGEN, Port Orange, FL, USA).
Microarray analyses, RNA amplification, labeling, hybridization and detection were performed following the protocols supplied by Applied Biosystems using the corresponding kits (Applied Biosystems).
Measurements of enzymatic activities of total antioxidant capacity (T-AOC), SOD, and malondialdehyde (MDA) contents were determined spectrophotometrically using the corresponding kits (Nanjin Jiancheng Bioengineering Institute, Nanjin, China) and following the manufacturer's protocol.
The contents of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in serum samples were determined by a GS200 Automatic Biochemical Analyzer (Shenzhen Genius Electronics Co., Ltd., Shenzhen, China) following the instructions of the corresponding reagent kit.
After electrophoresis and semi-dry blotting onto 0.45 µm nitrocellulose membranes (Whatman, Germany) the primary rabbit anti-ZO-1 antibody (1∶500; Invitrogen, Germany), rabbit anti-caspase 3 antibody (1∶1000, Cell Signaling, Germany) or rabbit anti-claudin-3 antibody (1∶1000; Invitrogen, Germany) were used in blocking reagent of the corresponding detection kit.
Serum urine nitrogen (BUN) and serum creatinine (Scr) were measured using the corresponding commercial kit (Leadman, Beijing).
