Their genes were synthesized, and expressed in E coli; corresponding His-tagged proteins were purified using the affinity chromatography.
A construct encoding the corresponding his-tagged version of the wild type enzyme (WT-his) was generated as a control.
For further analysis, we generated a fusion between the GLFG repeat domains of Nup49p and Nup57p, expressed the corresponding His-tagged fusion protein in Escherichia coli and purified it on a nickel chelate matrix.
These data indicate the mutation from glutamine to histidine occurred relatively late in the evolution of the MR. Interestingly, human AR has a corresponding His-885, which is found in mammalian AR but not in amphibian AR, which has a glutamine at this position [data not shown].
Each Nox candidate was preliminarily aligned with human Noxes to check whether the sequences conserved canonical regions required for O2- generation, such as the four heme-ligating histidines corresponding to His-101, His-115, His-209, His-222 of human Nox2 (GenBank™ No. NM_000388).
"His numbers were not corresponding with his previous numbers and you wondered, 'What's going on?"' Sanchez said.
He began corresponding with his friend Pete Fleming about his desire to minister in Ecuador, and in 1952 the two men set sail for Guayaquil as missionaries with the Plymouth Brethren.
He also began corresponding with his former wife, who during his incarceration had divorced him.
Locke evidently thought that the hypothesis corresponding to his scenario was true: that his scenario is possible.
Each message he sent via the mobile app, Facebook Messenger, came with a pushpin corresponding to his precise location, allowing me to trace his journey on a Bing map.
