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As shown in Fig. 5, the global radiographic score correlated positively with the arthritis score of the corresponding hind paw measured either at day 22 (Fig. 5a) or at day 195 (Fig. 5b) after AIA induction.
In a related lantern fly (Fulgoridae) that also jumps, the skeletal linkage between each hind coxa and the hinge of the corresponding hind wing, the pleural arch or Ugsprungsplatte [ 33, 34], is enlarged and specialised.
The mean intensity of the Doppler signal was quantified using proprietary software in a fixed ROI, encompassing the corresponding hind paw regions, normalized for the areas of the hind paws and expressed as numerical values (volts) to reduce the bias related to unavoidable anatomical and position variance.
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Likewise, significant correlation was found between the global radiographic score and paw diameter of the corresponding hind-paw measured either at day 22 (r = 0.627, p = 0.003) or at day 195 (r = 0.890, p <10-5, not shown) after AIA induction.
The signals corresponding to mid-hind brain and otic vesicle appeared to be similar in both control and ginkgotoxin-treated groups (Fig. 6A,B).
Diffuse uptake of the radiolabeled tracer was seen in the hind limbs corresponding to RAGE expression in the small arteries.
The shRNA against rat RalA (shRalA) and the two shRNAs against rat RalGDS (shRalGDS1 and shRalGDS2), targeting the nucleotide sequences AAGGCAGGTTTCTGTAGAA, GCCATGGACAAACACAACC and CTCTGCCGCCAACTATGAC, respectively, were prepared by inserting the corresponding oligonucleotides in the Hind III/Bgl II sites of a modified pSuper vector engineered to express the human Growth Hormone (hGH) [16].
As expected, rats only recovered minimal, presumably reflexive, movements of an occasional joint in the hind limbs, corresponding to a Basso Beattie Bresnahan locomotor score below 1 (Basso et al., 1995).
Perfusion in the ST lesions was on average elevated by 41 − 7.0 = 34 mL/min/100 cm3 or a factor of about 6. Table 4 Perfusion of ST lesions (right hind limb) and corresponding non-infected positions (left hind limb).
To study genetic variation in the covariance between two corresponding elements in fore- and hind limbs, we used ANCOVA, mapping loci for one phenotypic element while the second entered the model as a covariate.
To identify the origin of forces driving passive movements, we compared passive extensions and flexions in intact, denervated locust hind legs with corresponding movements measured after sequential ablation of the flexor and extensor tibiae muscles and tendons.
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Justyna Jupowicz-Kozak
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