Suggestions(1)
Exact(11)
The forward backward message is created by multiplying each corresponding forward and backward message value for each (k,m) pair, and normalizing the results as before.
Then 5 µl of the cDNA reaction mixture was used as a template in a 50 µl PCR amplification reaction mixture with corresponding forward and reverse primers (Table 2) and GoTaq DNA polymerase (Promega), as described by the supplier.
All PCR reactions were performed in a final volume of 50 µl, containing 5 µl of 10× PCR buffer (Invitrogen, Carlsbad, CA, USA) and 1 µl 100 mM deoxynucleoside triphosphates (Invitrogen), 1.7 µl of magnesium chloride, 1 µl of the corresponding forward and reverse primers at a concentration of 20 ng/µl and 0.5 µl of Taq DNA polymerase (Invitrogen).
The identity of the analysed coding genes and their corresponding forward and reverse primer sequences are listed in Table 1.
Rate constants are referred to as k+n and k−n and are assigned to the corresponding forward and reverse reactions.
Primers were then synthesized in 96-well microtiter plates with corresponding forward and reverse primers in alternate plates for simplified reaction set-up.
Similar(49)
If an inverted alignment region largely or completely overlapping the corresponding forward alignment, and if the inversion was aligned with higher similarity than the corresponding forward alignment based on following the criteria stated below, we defined the inverted region as an ultramicro inversion.
Deletion derivatives of the 1.5-kb 5′-flanking region of AtACBP6, 1.0, 0.6 and 0.3 kb in size were further amplified by PCR with corresponding forward primers ML1166, ML1167 and ML1168, and the reverse primer ML814.
Generation of b fragments was accomplished using the corresponding forward primer L61Xfor and reverse primer OAD21.
Three single mutant genes, AExynMC5T, AExynMP9S, and AExynMH14N, were constructed by PCR with the corresponding forward primers AM5-F, AM9-F, and AM14-F and a reverse primer AuX-R, respectively.
Forward pulling movements were elicited by a standardised stroke of the tail with serrated forceps, and the corresponding forward pulling tensions were recorded using a Grass Polyview recording system.
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