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Cells transfected with the corresponding empty expression vector served as the controls.
The total amount of DNA was adjusted to 0.8 μg per well by addition of each corresponding empty vector.
The CFRP/aluminum/steel specimens filled with honeycomb also crushed in a more stable progressive manner, exhibiting certain advantages over the corresponding empty counterparts.
A comparison with the corresponding empty tubular columns is performed, as the aim of the paper is to analyze the influence of each type of infill and establish the best option for practical application.
Plasmids pRTVnGFP-OsRac1, pRTVnRFP-SPIN6, and the corresponding empty vectors were used for co-localization assays.
The total amount of DNA in each transfection was kept constant by using the corresponding empty expression vectors.
Next, CON ovaries (left and right) were transplanted into the corresponding empty ovarian bursas of the DXR-treated female.
Total transfected DNA amount was set to an equal amount between all conditions by addition of the corresponding empty vector.
In each assay the total amount of DNA was held constant by addition of corresponding empty vectors.
First we infected Sp2cko/cko MEFs with a retroviral expression vector for Sp2 (pBABE-vSp2-puro), using infection with the corresponding empty vector (pBABE-puro) as a control.
For HEK293 cell transfection, cells (80% confluence) were transfected with an equal amount of total plasmid DNA (adjusted with the corresponding empty vector) by the calcium phosphate method.
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